Metabolites were extracted from the deep frozen cells by a pre-mixture of 300 µl methanol (final concentration ~80 %), 30 µl nonadeconoic acid methylester (2 mg ml-1 chloroform) and 30 µl of ribitol (0.2 mg ml-1), and D4-2,3,3,3-alanine (1 mg ml-1, standards in methanol). Each sample was mixed thoroughly at least 1 min for complete suspension, agitated 15 min at 70°C, brought to room temperature mixed with 200 µl chloroform and agitated again 5 min at 37°C.
我的社区
签到
赛默飞实验室产品焕新计划有奖调研！
【七月征文】不一YOUNG实验“猿”
报名开启：ICS2024第十三届光谱网络会议！
推荐收藏！盘点中药材及饮片检测解决方案
