Table of Contents
Preface, Pages xxiii-xxv
Chapter 1 - RNA and the Cellular Biochemistry Revisited, Pages 1-16
Chapter 2 - Transcription and the Organization of Eukaryotic Genes, Pages 17-31
Chapter 3 - Messenger RNA, Pages 32-46
Chapter 4 - Resilient Ribonucleases, Pages 47-66
Chapter 5 - RNA Isolation Strategies, Pages 67-113
Chapter 6 - The Truth About Tissues, Pages 114-137
Chapter 7 - Isolation of Polyadenylated RNA, Pages 138-162
Chapter 8 - Quality Control for RNA Preparations, Pages 163-178
Chapter 9 - Dot Blot Analysis, Pages 179-189
Chapter 10 - Electrophoresis of RNA, Pages 190-237
Chapter 11 - Photodocumentation and Image Analysis, Pages 238-260
Chapter 12 - Northern Analysis, Pages 261-284
Chapter 13 - Nucleic Acid Probe Technology, Pages 285-316
Chapter 14 - Practical Nucleic Acid Hybridization, Pages 317-332
Chapter 15 - Principles of Detection, Pages 333-360
Chapter 16 - Quantification of Specific mRNAs by Nuclease Protection, Pages 361-386
Chapter 17 - Analysis of Nuclear RNA, Pages 387-420
Chapter 18 - cDNA Synthesis, Pages 421-438
Chapter 19 - RT-
PCR, Pages 439-489
Chapter 20 - Quantitative
PCR Techniques, Pages 490-535
Chapter 21 - Transcript Subtraction Methods, Pages 536-554
Chapter 22 - mRNA Differential Display, Pages 555-587
Chapter 23 - High-Throughput Analysis of Gene Expression, Pages 588-600
Chapter 24 - RNA Interference: Targeted Gene Silencing, Pages 601-619
Chapter 25 - Genomes, Transcriptomes, Proteomes, and Bioinformatics, Pages 620-631
Chapter 26 - An RNA Paradigm, Pages 632-643
Epilogue - A Few Pearls of Wisdom, Pages 644-650
Appendix A - Maintaining Complete and Accurate Records, Pages 651-653
Appendix B - Useful Stock Solutions for the Molecular Biologist, Pages 654-660
Appendix C - Phenol Preparation, Pages 661-665
Appendix D - Disposal of Ethidium Bromide and SYBR Green Solutions, Pages 666-669
Appendix E - DNase I Removal of DNA from an RNA Sample, Pages 670-671
Appendix F - RNase Incubation to Remove RNA from a DNA Sample, Pages 672-673
Appendix G - Deionization of Formamide, Formaldehyde, and Glyoxal, Pages 674-675
Appendix H - Silanizing Centrifuge Tubes and Glassware, Page 676
Appendix I - Centrifugation as a Mainstream Tool for the Molecular Biologist, Pages 677-683
Appendix J - Trypsinization Protocol for Anchorage-Dependent
Appendix K - Isolation of High-Molecular-Weight DNA by Salting-Out, Pages 687-690
Appendix L - RNA Isolation from Plant Tissue, Pages 691-692
Appendix M - Electrophoresis: Principles, Parameters, and Safety, Pages 693-706
Appendix N - Polyacrylamide Gel Electrophoresis, Pages 707-709
Appendix O - Selected Suppliers of Equipment, Reagents, and Services, Pages 710-714
Appendix P - Useful SI Units, Page 715
Appendix Q - Common Abbreviations, Pages 716-717
Appendix R - Trademark Citations, Pages 718-721
Glossary, Pages 722-738
Index, Pages 739-767