刚开始接触相关文献，对其描述不太明白是什么意思，请高手们支招，谢谢！ “0 to 5min: 1% B;then a gradient is started to 1.4%B @ 27.5min,followed by a sharp increase to 10%B in 1min. hold @ 10%B for 10min, back to 1%B in 1 min, reequilibrate for 10 min at 1%B.The wash phase(@ 10% propanol) should be done in backflush mode,i.e. reverse the direction of flow if possible (depends on the layout of your LC, but if you still have to purchase one....). Depending on what type of pump you have, check whether you can purchase "normal phase" seals for the pump and install them on the pumphead that pumps the hexane. It will save you serious downtime for constantly changing seals. make sure you have some sort of in-line filter between your column and MSinlet, and keep clean spares handy.Depending on the type of LC, check whether you have a no-waste injection mode or partial loop fill. Obviously the firse option is preferred as this prevents using more sample than actually injected.”
Depending on what type of pump you have, check whether you can purchase "normal phase" seals for the pump and install them on the pumphead that pumps the hexane. 根据泵的类型，确认是否可以购买“正相”密封垫并安装在泵正己烷的泵头 It will save you serious downtime for constantly changing seals. 这可以节省很多用于更换密封垫的停工时间 make sure you have some sort of in-line filter between your column and MSinlet, and keep clean spares handy. 确保柱子和入口间有在线过滤器，并存有干净备用品 Depending on the type of LC, check whether you have a no-waste injection mode or partial loop fill. Obviously the firse option is preferred as this prevents using more sample than actually injected.” 根据液相色谱仪的型号，确认配备的是“不浪费样品进样方式”或“部分充满定量环进样方式”。显然更倾向于选择第一种进样方式，因其可以避免使用比实际进样多的样品
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