主题：【原创】生物碱和苷类同时分离

使用动态涂层技术。

出峰拖尾说明你的生物碱和毛细管内壁是有一定相互作用的，你可以用动态涂层的方法去除这种吸附。

出峰拖尾说明你的生物碱和毛细管内壁是有一定相互作用的，你可以用动态涂层的方法去除这种吸附。

你可以尝试商品化的试剂盒来解决分离和峰拖尾的问题：

Separation of Neutral Compounds by CE using CElixir-SDS™
Neutral compounds normally migrate with the Electro-Osmotic Flow (EOF) in CZE. Using the proper buffer additives, MEKC can employed and neutral compounds can be separated along with charged molecules. This pre-made 搗alidated? kit makes MEKC easy to perform, reliable and a powerful addition to any laboratory taking advantage of the power and advantages of CE.

Method Conditions:
Catalog No.: 06200-50
Supplied Buffers and Solutions were used without modification
Voltage: 25kV
Capillary: MicroSolvCE™ Bare Fused Silica, 75祄 x 60cm
Injection: 5 sec, 0.5psi followed by post injection Water Plug
Run Buffer: CElixir-SDS Accelerator™ 50-50 Borate pH 9.2
Detection: UV, 200nm
Polarity: Normal

Sample Preparation:
A standard Test Mix of Caffeine, Sorbate, Benzoate, Saccharine, Acesulfame and Quinine was used.

Discussion and Rationale:
The CElixir-SDS™ kits use the patented dynamic coating technology of CElixir™ which makes getting results easier and more reliable. Precision through stabilization of the EOF and control of the SDS allows these kits to be used by beginners as well as experienced experts. No buffer preparation and therefore less variation in critical CE parameters also makes these kits the choice of routine as well as research laboratories.

原文由 heguang(heguang) 发表:
出峰拖尾说明你的生物碱和毛细管内壁是有一定相互作用的，你可以用动态涂层的方法去除这种吸附。

那可不一定，总说生物碱拖尾是因为吸附管壁，可我没有用任何涂层，依然做出很漂亮的峰。有时候拖尾可能是柱子不太行，换根柱子，可能就有很大的改善

原文由 april110016(april110016) 发表:

原文由 heguang(heguang) 发表:
出峰拖尾说明你的生物碱和毛细管内壁是有一定相互作用的，你可以用动态涂层的方法去除这种吸附。

那可不一定，总说生物碱拖尾是因为吸附管壁，可我没有用任何涂层，依然做出很漂亮的峰。有时候拖尾可能是柱子不太行，换根柱子，可能就有很大的改善

拖尾是很明显的吸附现象，你做的生物碱有很漂亮的峰，说明你的生物碱没有吸附情况，不代表别人的样品会产生吸附。