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| Column | Cogent Diamond Hydride™, 4µm, 100A | ||||||||||||||||
| Catalog No. | 70000-15P-2 | ||||||||||||||||
| Dimensions | 2.1 x 150 mm | ||||||||||||||||
| Solvents | A: DI water + 0.1% ammonium formate pH 7.2 B: 90% acetonitrile + 10% DI water + 0.1% ammonium formate pH 6 | ||||||||||||||||
| Mobile Phase | Gradient:
| ||||||||||||||||
| Post Time | 5 min. | ||||||||||||||||
| Flow Rate | 300 µL/min. | ||||||||||||||||
| Compounds | 1. UDP glucose - the monitored MRM transitions were m/z 565 to m/z 323 2. UDP hexanolamine (internal standard) - the monitored MRM transitions were m/z 502 to m/z 258 (MRM � multiple reaction monitoring in LC/MS/MS) | ||||||||||||||||
| Detection | ESI � neg - Agilent 6410 Triple Quadrupole LC/MS |
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