主题：[转贴]：读书笔记－Solvent Guidelines

Do not use any of the following solvents in the Waters Reagent Manager because they damage PEEK components.
• Methylene chloride
• Tetrahydrofuran (THF) at greater than 10% (v/v)
• Freon
• Concentrated sulfuric acid (H2SO4)
• Concentrated nitric acid (HNO3)
Also keep in mind the following solvent guidelines:
• Use methanol to prime a new pump or a pump that has been stored for an extended period.
• Use an intermediate solvent when you change between solvents that are not miscible.
• Always keep the solvent level in the reservoir at least 4 inches ( 10cm) higher than the pump inlet. Do not place solvent reservoirs on top of the Reagent Manager.
• Use only filtered, degassed solvents.
• After using a buffer, purge the pump with HPLC-grade, distilled, or deionized water to prevent crystallization and damage to the piston or seal.
• Know the properties of the solvents under different pressures.
• Dedicate a solvent supply tube and filter assembly for each solvent. If this is not possible, purge the tube and filter of any remaining solvent before you use a new  solvent.
• To prevent cross-contamination of solvents, dedicate a set of glassware for preparing, storing, and supplying each solvent. Once a container has been used for a solvent, do not use it for any other solvent unless it has been thoroughly cleaned.