107.9
大孔树脂处理对芫花药材HPLC
指纹图谱的影响 【作者】 李娆娆; 王彩芳; 黄兰岚; 钟利群; 原思通;
【Author】 LI Rao-rao~ 1 , WANG Cai-fang~ 1,2 , HUANG Lan-lan~ 1,3 , ZHONG Li-qun~4, YUAN Si-tong~1(1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;2. College of Pharmacy, Zhengzhou University, Zhengzhou 450052, China; 3. Jiangxi College of Traditional Chinese Medicine, Nanchang 330006, China; 4. Dongzhimen Hosptal, Beijing University of Traditional Chinese Medicine, Beijing 100700, China)
【机构】 中国中医科学院中药研究所; 郑州大学药学院; 江西中医学院; 北京中医药大学东直门医院;
【摘要】 目的:考察以大孔树脂法制备供试品溶液,对芫花药材高效
液相指纹图谱的影响。方法:对17批药材进行HPLC指纹图谱分析。供试品溶液制备方法为甲醇提取,提取液回收试剂至干,残渣水溶解后经大孔树脂柱层析,收集20%、50%和95%乙醇洗脱物。以高效
液相色谱法对各批药材的甲醇提取液及各洗脱物进行指纹图谱分析。色谱条件:Diamonsil C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇、水两相梯度洗脱,流速1mL·min-1,柱温35℃,检测波长332nm,记录时间40min。结果甲醇直接提取所得到的共有峰为7个,而经大孔树脂柱层析后50%乙醇洗脱物得到共有峰11个。结论大孔树脂能够富集成分,在指纹图谱中显示更多的色谱峰,使对照图谱的指纹性增加,采用该方法来制备供试品溶液有较为广阔的应用前景。 更多还原
【Abstract】 Objective:To analyze the effect on HPLC fingerprint of Flos Genkwa which samples prepared by macroporous resin. Method: The fingerprints of 17 batches of Flos Genkwa were determined. The samples were extracted with methanol, then solvent were distilled off underreduced pressure. The residue were solved by water. Macroporous resin column chromatography was used for separating the water solution. Three parts of eluates, 20%, 50% and 95% ethanol eluate were obtained, separately. Then they were anal... 更多还原
【关键词】 芫花药材; 大孔树脂法; 高校
液相; 指纹图谱;
【Key words】 Flos Genkwa; macroporous resin preparation method; HPLC; fingerprint;
【基金】 国家科技基础条件平台工作(2003DIATJ031)