【Author】 WANG Li-sheng~1,ZHAN Jian-kun~2,ZHOU Li-ling~1(1.College of Traditional Chinese Materia Medica,Guangzhou University of TCM,Guangzhou 510405,China;2.Grade 2002 Undergraduate of Pharmaceutical Engineering,College of Traditional Chinese Materia Medica,Guangzhou University of TCM,Guangzhou 510405,China)
【Abstract】 ObjectiveTo establish the determination method for osthole in Dieda Huoluo Microemulsions.MethodsHPLC was used with DiamonsilC18 column（250 mm×4.6 mm,5 μm）,detection wavelength at 322 nm,mobile phase: methanol-water（80∶20）,flow rate: 1ml/min.ResultsOsthole has good linear relationship between 0.0420.336 μg,r=0.999 7,the recovery of osthole was 100.91% and RSD was 2.42%（n=6）.ConclusionThe method is simple,sensitive and reliable to carry out to control the quality of this pr... 更多还原 【关键词】 跌打活络微乳； 高效液相色谱法； 蛇床子素； 【Key words】 Dieda Huoluo Microemulsions； HPLC； Osthole； 【基金】 国家自然科学基金“中药经皮给药系统局部药动学研究”(No.30371783)
【Abstract】 Objective To establish a method for determining the content of Danshensu in Rukuaixiao Mixture by HPLC.Methods The determination was carried out with Diamonsil-ODS C18 column;the mobile phase consisted of cetonitrile-methanol-water-glacial acetic acid（0.5∶8∶92∶1）,and the detection wavelength was 280 nm.Results The average recovery of standard addition was 99.87%,RSD=1.73%;there was a good linear relationship between the absorption area value and the concentration in the range of 0.106... 更多还原 【关键词】 乳块消合剂； 高效液相色谱法； 丹参素； 【Key words】 Rukuaixiao mixture； HPLC； Danshensu；
【Abstract】 Objective:To establish the HPLC method for detemination of strychnine and brucine in compound Fengshi Pills.Methods:HPLC was performed on Diamonsil C18（4.6 mm×200 mm,5 μm）with methanol-acetonitrile-HAC（45∶30∶25∶0.5）as mobile phase.Detecting wavelength was 254 nm and flow rate was 1.0 ml/min.Strychnine and Brucine in compound Fengshi Pills were extracted by silica gel solid-phase extraction method.Results:Linear ranges of Strychnine and Brucine were 10～100 μg/ml and 6 μg/ml～60 μg/ml re... 更多还原 【关键词】 高效液相色谱法； 复方风湿丸； 士的宁； 马钱子碱； 含量； 【Key words】 Compound Fengshi Pills； Strychnine； Brucine； Content；
【Abstract】 Objective:The effect of ehinacoside content of Cistanche deserticola was studied by adding precursors and revulsant to hosts.Set up the method on determination of the content of echinacoside in herbs of Cistanche deserticola by HPLC.Method:Chromatographic conditions were available as follows DiamoNsil Dilcma C18(4.6mm×250mm,5μm),l.5% acetate solution as mobile phase A,methanol as mobile phase B,flowrate was 0.5mL/min,and detecting wavelength was 335 nm,room temperature.Result:The linear correlat... 更多还原 【关键词】 肉苁蓉； 前体物质； 诱导剂； 松果菊苷； 高效液相色谱法； 【Key words】 Cistanche deserticola； precursors； revulsant； echinacoside； HPLC； 【基金】 新疆大学自然科学基金项目(编号:XY080110)资助
【Abstract】 Objective Herba Epimedii was fermented with spore suspension of Aspergillus niger and the method for detecting icariin during fermentation was established.Methods The solid-state fermentation condition was 30℃ for 7 days with spore density of 1.8×104/mL.The water reflux,ethanol reflux and ultrasonic water,ultrasonic ethanol extraction were applied for the extraction of icariin from Herba Epimedii.High performance liquid chromatography(HPLC) was employed to determine the icariin.Conclusion The su... 更多还原 【关键词】 淫羊藿； 固体发酵； 黑曲霉； 高效液相色谱； 【Key words】 Herba Epimedii； solid-state fermentation； Aspergillus niger； HPLC； 【基金】 北京市教育委员会科技发展计划资助项目(KM200911417011)
【Author】 Jiatai Zhao1, Wanli Ji2(1,Shanxi Taihang Pharmaceutical Co.Ltd Changzhi 0460002,Shanxi Changzhi Institute for Drug Control Changzhi 046000)
【机构】 山西太行药业股份有限公司； 山西省长治市药品检验所；
【摘要】 目的:用高效液相色谱法测定注射用法莫替丁中有关物质的含量。方法:采用Diamonsil C 18色谱柱;以0.01mol.L-1庚烷磺酸钠溶液-乙腈-甲醇(25∶6∶1),用冰醋酸调节pH至3.9;流速:1.0ml/min,检测波长为267nm。结果:有关物质的浓度在106～317μg.ml-1范围内均与峰面积呈良好的线性关系,r为0.9712,法莫替丁与有关物质能有效分离,有关物质的最低检测限(S/N=3)为0.02 ng.ml-1。结论:此方法简便、快速、准确,可用于该药有关物质的检查。 更多还原
【Abstract】 Objective: An HPLC method for the determination of relative substances in famotidine for injection was established.Methods: Diamonsil C 18 column;the mobile phases consisted of 0.01 mol ? L-1 sodium heptanesulfonate solution- acetonitrile-methanol (25∶6∶1)(adjust pH to 3.9 with Acetic acid ); The flow rate was 1.0ml/min, detecting wavelength was267nm. Results: Thelinear range of relative substances was106～317μg?ml-1,γ=0.9712, famotidine And relative substances can be effectively separated,The lo... 更多还原 【关键词】 高效液相； 法莫替丁； 有关物质； 【Key words】 HPLC； famotidine； relative substances；
【Abstract】 Objective To establish a high-performance liquid chromatography-electrospray mass spectrometry(HPLC-MS) method for the determination of docetaxel in rat plasma.Methods The HPLC-MS method was performed on Diamonsil C18 column(4.6 mm×200 mm,5 μm) at 25 ℃,taking acetonitrile-0.2% formic acid(V∶V=66∶4) as mobile phase with flow rate of 1.0 mL·min-1;the injection volume was 20 μL.Acquisition was performed with the selected ion reaction monitoring mode(SRM) after the ionization in the electrospray ion... 更多还原 【关键词】 多西他赛； 高效液相色谱-质谱联用法； 含量测定； 【Key words】 doctaxel； high-performance liquid chromatography-electrospray mass spectrometry； content determination； 【基金】 辽宁省科技计划项目(2007102071)
【Abstract】 Objective To establish an HPLC method for simultaneous determination of rhein,emodin,chrysophanol and physcion in Shudan(traditional Chinese medicines)capsules.Methods The chromatographic method was applied on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with methanol-water-phosphoric acid(V∶V∶V=84∶16∶0.1) as the mobile phase.The flow rate was 1.0 mL· min1 and column temperature was 35 ℃.The detection wavelength was set at 254 nm.Results The calibration curve was linear within the range of 0.220 0... 更多还原 【关键词】 舒胆胶囊； 大黄酸； 大黄素； 大黄酚； 大黄素甲醚； 高效液相色谱法； 【Key words】 Shudan capsule； rhein； emodin； chrysophanol； physcion； HPLC；
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