【Abstract】 Objective To establish an HPLC method for simultaneous determination of palmatine hydrochloride and berberine hydrochloride in Qinlian tablets.Methods The separation was performed on a Diamonsil C18(4.6 mm ×200 mm,5 μm)column.The mobile phase was composed of 50 mmol·L-1 NaH2PO4(pH 4.6) and acetonitrile at the rate of V∶V=72∶28.The column temperature was set at 35 ℃ and the flow rate was 1.0 mL·min-1,with UV detection at 343 nm.Results The numbers of theoretical plates calculated by palmatine hyd... 更多还原 【关键词】 芩连片； 高效液相色谱法； 盐酸巴马汀； 盐酸小檗碱； 【Key words】 Qinlian tablet； HPLC； palmatine hydrochloride； berberine hydrochloride；
【Abstract】 Objective To establish an RP-HPLC method for determination of quercetin in Kanlisha(traditional Chinese medicine).Methods Chromatagraphy was conducted on a Diamonsil C18 column(250 mm×4.6 mm,5 μm) at 25 ℃ and 370 nm.The mobile phase was acetonitrile-0.3% phosphoric acid aqueous(V∶V=33∶67) at a flow rate of 1.0 mL·min-1.Results The calibration curve was linear in the range of 6.312 5-101.0 mg·L-1(r=0.999 5);The average recovery was 95.1% and RSD was 3.3%(n=9);The precisions RSD was 2.4%(n=5).Conc... 更多还原 【关键词】 高效液相色谱法； 坎离砂； 槲皮素； 【Key words】 HPLC； Kanlisha； quercetin；
【Abstract】 Objective To develop a method of high performance liquid chromatography for the analysis of fingerprint of Rhizoma Drynariae.Methods The analysis was performed on DiamonsilTM C18 column(200 mm×4.6 mm,5 μm),with a gradient acetonitrile-water(adjusted to pH 3.0 with acetic acid) as mobile phase at a flow rate of 1.0 mL·min-1,and at a column temperature of 25 ℃.The UV detector was set at 270 nm.Results The fingerprint of Rhizoma Drynariae was established and eight common peaks were displayed in the... 更多还原 【关键词】 骨碎补； 高效液相色谱法； 指纹图谱； 质量控制； 【Key words】 Rhizoma Drynariae； HPLC； fingerprint； quality control； 【基金】 国家自然科学基金资助项目(30472168)
【Abstract】 Objective To develop an HPLC method for the simultaneous determination of chlorogenic acid and protocatechuic acid in Yinshanlian(traditional Chinese medicines) granules.Methods Chromatography was performed on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with the mobile phase of acetonitrile-0.2% phosphoric acid solution(V∶V=10∶90).The detection wavelength was set at 285 nm and the column temperature was 30 ℃.Results The linear calibration curve were obtained in the concentration range of 4.80-43.... 更多还原 【关键词】 茵山莲颗粒； 绿原酸； 原儿茶酸； 高效液相色谱法； 【Key words】 Yinshanlian granules； chlorogenic acid； protocatechuic acid； HPLC；
【Abstract】 Objective To develop an HPLC method for the determination of pantoprazole in human plasma.Methods After a simple liquid-liquid extraction,pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm.The mobile phase consisted of methanol-water(V∶V=60∶40).Results The linear calibration curve was obtained in the concentration range of 0.02-5.0 mg·L-1.The lower limit of quantification was 20 μg·L-1.The intra-and inter-day precision(RSD) were... 更多还原 【关键词】 泮托拉唑； 高效液相色谱法； 药物动力学； 血药浓度； 【Key words】 pantoprazole； HPLC； pharmacokinetics； plasma concentration；
【Author】 HE Yang1,WANG Dong-mei1,DONG Zhen-min2,SHEN Tao1,LUAN Shuang1,ZHAO Huai-qing1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Department of Health Care Managent of Liaohe Oilfield,Panjin,124010,China)
【Abstract】 Objective To develop a method for determination of scutellarin and salvianolic acid B in Dandengtongnao(traditional Chinese medicines) capsules by HPLC.Methods The analysis was carried on a Diamonsil C18(200 mm × 4.6 mm,5 μm,Dikma)column.The mobile phase was composed of 5% acetic acid(A) and acetonitrile-methanol(1∶1) with gradient elution(0-15 min,30%-36% B,15-25 min,36% B,25-30 min,30%-36% B,volumn fraction),the flow rate was 1.0 mL·min-1.The detection wavelength was set at 280 nm and column t... 更多还原 【关键词】 丹灯通脑胶囊； 野黄芩苷； 丹酚酸B； 高效液相色谱法； 【Key words】 Dandengtongnao capsules； scutellarin； salvianolic acid B； HPLC；
【Abstract】 Objective To establish the quality standard of Weitongping syrup(Chinese meteria medica).Methods A HPLC method was discribed for the determination of chelerythrine with UV absorbance detection.The separation was performed by using a Diamonsil ODS column with a mobile phase consisting of methanol-acetonitrile-water-triethylamine(V∶V∶V=70.0∶∶10.0∶20.0∶0.2).The UV detector operated at 270 nm.A TLC method was used for distinguishing Chelidonium majus L.Results The negative comparison displayed no di... 更多还原 【关键词】 胃痛平糖浆； 质量标准； 白屈菜红碱； 高效液相色谱法； 【Key words】 Weitongping syrup(Chinese meteria medica)； quality standard； chelerythrine； HPLC；
【Abstract】 To meet the requirement of reference materials in quality evaluation and detection of propolis,this paper preliminary explored active ingredients reference materials including ferulic acid,chrysin and apigenin in propolis.The purity of active ingredients in propolis was determined by high-performance liquid chromatography(HPLC) with platisil ODS C18 column.The mobile phase is the mixture of methanol-phosphate(pH 3.0) system with the flow rate of 1.0 mL/min.The detection wavelength was 325 nm(fer... 更多还原 【关键词】 蜂胶； 活性成分； 标准物质； 高效液相色谱； 【Key words】 propolis； active ingredients； reference materials； high-performance liquid chromatography； 【基金】 国家科技基础性工作专项(2008FY130200)资助
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