原文由 klaidy 发表:
Spectrophotometer 分光光度计
Measuring transmittance within a fairly narrow frequency band requires a suitable spectrophotometer in which the wavelength of the light source can be varied or restricted by the use of a 580-nm filter or a 530-nm filter for reading the absorbance in a tube assay.For the latter purpose,the instrument may be arranged to accept the tube in which incubation takes place (see Turbidimetric Assay Receptacles),to accept a modified cell fitted with a drain that facilitates rapid change of content,or preferably,fixed with a flow-through cell for a continuous flow-through analysis;set the instrument at zero absorbance with clear,uninoculated broth prepared as specified for the particular antibiotic,including the same amount of test solution and formaldehyde as found in each sample.
NOTE—Either absorbance or transmittance measurement may be used for preparing inocula.
在一个相当狭窄的频率波段中测量透光率要求一个适合的分光光度计,其光源的波长可以改变或者限制通过使用580nm或者530nm的过滤器来读取在管试验中。为了进一步的开展,该仪器可以被安排在接收该管在接种培育发生期间(见浊度法仪器),使用一个变形细胞具有排水功能的过滤器以便能快速促成改变内容物的量。或者更好的,通过持续的流通分析固定一个流通细胞,清楚的确定该仪器于0,对于制备确定的抗生素时不接种肉汤包括相同数量的试验溶液和当用甲醛溶解每个样品的时候。
注: 制备接种体的吸光度和透光率。
Suggestion---
For the latter purpose : 对于後者 (i.e. restricting the wavelength of the light source)
set the instrument at zero absorbance with clear,uninoculated broth …… : 以clear,uninoculated broth ……(为参考值?)将仪器的absorbance调为零
NOTE—Either absorbance or transmittance measurement may be used for preparing inocula : 注: 制备接种体的吸光度和透光率。
(Did 楼主translate this with computer software?)注: Absorbance or transmittance 皆可用于制备inocula