我也来参加,呵呵,不过英语实在不是很好,肯定有很多语法问题,请大家包涵啦。
Now I’m working in a clinical pharmacological laboratory of a provincial hospital. The primary services provided by our laboratory are study of clinical pharmacokinetics and drug metabolism, especially the clinical pharmacokinetics of Traditional Chinese Medicine (TCM) and the bioavaila-bility (BA) and bioequivalence (BE) research of new drugs in healthy volunteers.
My job focuses on the determination of drug concentrations in human plasma using precise instrument after they took test preparations.In technical words, it is called biopharmaceutical analysis. The main instruments to determine drugs in biological specimen used in our laboratory are high performance liquid chromatography (HPLC), liquid chromatography-mass spectrography (LC/MS) and liquid chromatography-tandem mass spectrography (LC/MS/MS). The table below listed the main precise instruments of our laboratory.
Instrument Numbers
Waters 2695 HPLC 2
Agilent 1100 HPLC 2
Waters ZQ 2000 LC/MS 1
Waters Quattro Micro LC/MS/MS 1
Waters Quattro Primer UPLC/MS/MS 1
AB API4000 LC/MS/MS 1
Once I accepted a determination project, I need to look up the literature, develop an analytical method, finish the determination of all samples (always nearly to 1000) and complete the Summary Report within about several months. And developing an analytical method was the key procedure of the whole process.
There were no government standards in my working field. Thus looking up the literature was the first step when I began my task, and its result determined if I need to develop a new assay or not. Inevitably, I usually encountered many literatures written in English, some of which I got through the internet, such as this web by asking for help. Nevertheless, my written English is so poor that please forgive my terrible English in this article. Let’s get to the point.
If I couldn’t find any HPLC, LC/MS or LC/MS/MS method to determine my target compound in the literature, or the method was not suitable to my compound, I need to develop a brand-new assay.
According to the sensitivity request, I should set up a new determination method, with proper fore-treatment, suitable equipment and optimized
chromatographic and mass spectrographic conditions, which could cost me several days to weeks. The person with rich experience could greatly shorten the time of this procedure. I always came to this forum since I came across it in 2006. Not only could I learn much expertise, but also I knew a lot of friends here. Though we didn’t ever meet each other, we discussed questions, worked out a solution together and shared successful happiness with each other. To publish methodology articles in professional magazines, especially in SCI magazines, was the best affirmation of this work. But my English was so poor that I would spend more time on English learning. Sometimes, I just need to modify some procedure and conditions of the reported assay according to my lab’s current qualification. In a word, method developing is a challenging and enjoyable work.
After setting up a stable assay, I would apply it in my project. I determined the drug concentrations in human plasma with this method. There were always up to 1,000 samples and it usually cost me several weeks. Keeping this procedure smoothly requested us more carefully in sample fore-treatment, column washing and instrument utilization (following the standard of procedure, SOP). The signals of MS was always changed even if had been repaired from a minor problem. Therefore, serious working attitude could keep every thing in good condition and brought us with good reproducibility and data.
Completing the Summary Report was the last work I should do. Data analysis with professional soft ware, such as BAPP and DAS, and filling the data in the report platen were what I need to do. From the results, I could know if the new drug was bioequivalent to the reference preparation.
What I’m doing now is a necessary procedure of an imitated new drug.
终于写完啦,大家凑合看吧,本人好久没写这么长的英文了,呵呵