for your reference as the following. sorry just in English. One factor in creating an accurate calibration and sample quantitation is to be sure that your peaks are integrated correctly. There are two integrators to choose from when using your MS ChemStation software. They are: 1) the RTE Integrator or 2) the ChemStation Integrator. Both have their advantages and limitations. The RTE Integrator is a simple and fast integrator. The ChemStation Integrator can handle complex chromatograms, but is slower than the RTE Integrator. The RTE Integrator is recommended for routine quantitative work and the ChemStation Integrator is recommended for routine qualitative work. Data point sampling selects which data points are used in calculating slope sensitivity. With the default selection of 1 every data point is used. Select 2 for every other point, 3 for every third, etc. Range is from 1 to 9. Negative values are not allowed. Sampling should normally be set at 1 for scanned capillary column data. For SIM data or for very noisy data (anytime the integrator shows a tendency to “split peaks”), this parameter is frequently 2 or 3. This parameter was called bunch in earlier versions of RTEINT. Qualitative Data Analysis RTE Integrator 8 The smoothing checkbox turns on an additional filter that acts on the data when the first derivative is being taken to determine the start/stop points. Default = not selected. You can select Smoothing only when you also select 5-point digital filtering. Use detector smoothing to improve results with noisy data and anytime the integrator shows a tendency to “split peaks”. A split peak is a single chromatographic event or peak that is reported as two peaks because of a minor perturbation. This is the same filter enabled in previous versions of RTEINT when negative values were entered for bunch. Detection filtering is used to improve detection of noisy chromatograms. It can improve results with SIM data as well as with broader peaks. This parameter lets you select the number of data points to be included in a moving weighted average. Proper parameter selection depends on how narrow or broad typical peaks are and should be done in conjunction with data point sampling. Start threshold is rarely changed. The default setting of 0.2 is optimal for most chromatograms. A peak is detected or started when the first derivative calculation exceeds the starting threshold, which is in part determined by the parameter setting. The stop threshold determines when a peak ends. This threshold can be adjusted to vary the amount of a tailing peak that is included in the integrated peak area. Higher values increase the amount of a tailing peak that is integrated. Noise in the data can cause integration to terminate prematurely. In such cases, try raising the value to 0.1 or 0.2 to extend the end of the peak. Baseline reset (# points) > is the number of scans that must separate two adjacent chromatographic peaks in order for the coordinates of start and end points of the peaks to be used to define the baseline. If two or more peaks are separated by less than this value, the baseline is drawn below the abundance at the start or stop of the peak. If leading or trailing edge < is used in conjunction with Baseline Preference to determine whether a drop to baseline or a tangent from start to stop occurs at a peak. The parameter is a measure of the difference in amplitude of a peak’s start / stop points expressed as a percentage of total peak height. Note that in RTE this parameter is also known as valley. Baseline Preference is used in conjunction with If leading or trailing edge to cause a baseline drop or a tangent from start to stop of a peak. In the default case of If leading or trailing edge < 100%, then Baseline drop else tangent, baseline drop is used for all peaks because all peaks satisfy the condition. In the same way, if you choose Tangent else baseline drop, all peaks would have a tangent from start to stop. If you enter a value other than 100%, then both baseline drops and tangents are possible in a given chromatogram. For example: If leading or trailing edge < 20%, then Tangent else baseline drop, would cause peaks with less than 20% difference in abundance from start to stop to have the baseline drawn from start to stop. Other peaks would have a drop from start or stop to a horizontal baseline. Qualitative Data Analysis RTE Integrator 9 Minimum peak area is the area threshold. For target compound analysis, Area counts is normally the better selection. % of largest peak should not be used for target compound detection because the sensitivity of the detection phase detects many noise peaks if the target compound is not present. In other words, if the target compound is not present, the system is forced to integrate noise. These noise peaks could appear as false positives in the quantitation report. Area counts affects the peak detector. In this case, the peak detector examines the raw area of each integrated peak to determine if the peak should be retained and submitted to the baseline allocation algorithm. With peak location you can select whether retention time is reported at the top of a peak or to the centroid (center of the effective area). The default location is top. This option is useful for broad noisy peaks. Maximum number of peaks to report. Range is 1 to 250. The peaks reported are those with the largest areas. Use the Save button to save the current RTE integration parameters to a *.P file associated with a particular method. Use the Load to load parameters from the macro file (*.P) that has RTEintegration parameters for the method.