Calibrating the Conductivity Cell When to Calibrate • After every 6 months of use
NOTE Do not use this procedure to calibrate a new cell.
1. Open the ICS-1000 Wellness Panel in Chromeleon 2. On the Wellness Panel under Electric Conductivity Cell Calibration, click Offset Cal. 3. When the offset calibration is complete, click Slope Cal on the Wellness Panel. 4. Disconnect the pump output line from the injection valve. 5. Disconnect the line from the suppressor ELUENT OUT port to the cell inlet and connect the pump output line directly to the cell inlet. 6. Verify that there is a minimum of 7 MPa (1000 psi) of backpressure. 7. On the Wellness Panel under Conductivity Cell Calibration, click Cell 35 C. Allow the cell to reach this temperature and then wait an additional 5 minutes to let it stabilize. 8. Select 1.00 mL/min to begin pumping 1.0 mM KCl through the cell at 1.0 mL/min. 9. Wait until the total conductivity reading stabilizes (in about 15 minutes) and then select Calibrate on the Wellness Panel. After calibration, the conductivity reading should be 147.00 ± 2 μS and the cell constant should be between 120 and 180. If this is not the case, contact Dionex for help. 10. Click Log to record the new calibration values in the Audit Trail. 11. Flush the KCl solution from the system by pumping deionized water through the cell. When the conductivity drops to less than 1 μS/cm, stop the pump. 12. Reconnect the pump to the injection valve and reconnect the line from the suppressor to the cell inlet.
Items Needed Description 1.0 mM KCl solution ---- Prepare by dissolving 0.07456 g of reagentgrade KCl in 1 liter of 18-megohm DI water. Backpressure tubing to provide at least 7 MPa (1000 psi)-----Use 0.076-mm (0.003-in) ID yellow PEEK tubing (P/N 049715).
Thanks !But chinese is easy to read !
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suppressor加 5积分,
2经验,加分理由:Thanks for sharing!