Agilent
Bonded Phase Pore Size Surface Area pH Range Functionality Max Pressure
ZORBAX 300 SCX 300A 50 m2/g 2 - 6.5 Sulfonic Acid 350 bar
ZORBAX SAX 70A 300 m2/g 2 - 6.5 Quaternary Amine 350 bar
Sigma
65721-U
Supelco
Discovery® Zr-SAX HPLC Column
3 μm particle size, L × I.D. 15 cm × 4.6 mm
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65721-U 11561.94 Date not Available
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Description
Features and Benefits • Efficient strong anion-exchanger useful for inorganic and organic anions. Stable from pH 1-12.
• Ideal for the separation of water-soluble vitamins.
• Useful for the separation of bio-molecules such as nucleotides, nucleosides, oligonucleotides, oligodeonucleotides, amino acids and peptides.
• High anion-exchange capacity that can be controlled by the amount of polymer deposited on the porous zirconia substrate.
• Does not shrink or swell as a function of anionic strength or organic modifier content of the mobile phase.
• Extremely stable amino phase for normal phase separation of carbohydrates.
• Thermally stable up to 80oC, which causes different selectivity and high speed separations with lower ionic strength mobile phases. This is very important in the preparation of RNA and DNA samples used for further studies.
• Mixed-mode separation modes may be exploited to optimize separations, including Lewis acid-base interactions, hydrophobic interactions and ion-exchange interactions. These modes may be attenuated by adjusting the strong Lewis base content, organic content and ionic strength of the mobile phase, respectively.
Legal Information Discovery is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Properties
extent of labeling 25% Carbon loading
limit <80 °C temp. range
L × I.D. 15 cm × 4.6 mm
matrix zirconia spherical, porous particle platform
matrix active group polyethyleneimine bonding phase
particle size 3 μm
pore size 300 Å
surface area 30 m2/g
operating pH range 1 - 12