主题:【讨论】色谱柱选型

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要求:

1.分离模式:离子交换
2.分离对象:氨基酸、氨基酸类似物、草铵膦
3.可供选择公司:Agilent、Waters、Sigma等
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tomdreams
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Agilent
Bonded Phase    Pore Size    Surface Area    pH Range    Functionality    Max Pressure
ZORBAX 300 SCX    300A    50 m2/g    2 - 6.5    Sulfonic Acid    350 bar
ZORBAX SAX    70A    300 m2/g    2 - 6.5    Quaternary Amine    350 bar

Sigma
65721-U
Supelco

Discovery® Zr-SAX HPLC Column
3 μm particle size, L × I.D. 15 cm × 4.6 mm

Price and Availability
Product
Number    Your Price
RMB    Available to Ship    Quantity    Actions
65721-U    11561.94    Date not Available   
details...                    

   

Specifications
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Description
Features and Benefits    • Efficient strong anion-exchanger useful for inorganic and organic anions. Stable from pH 1-12.
• Ideal for the separation of water-soluble vitamins.
• Useful for the separation of bio-molecules such as nucleotides, nucleosides, oligonucleotides, oligodeonucleotides, amino acids and peptides.
• High anion-exchange capacity that can be controlled by the amount of polymer deposited on the porous zirconia substrate.
• Does not shrink or swell as a function of anionic strength or organic modifier content of the mobile phase.
• Extremely stable amino phase for normal phase separation of carbohydrates.
• Thermally stable up to 80oC, which causes different selectivity and high speed separations with lower ionic strength mobile phases. This is very important in the preparation of RNA and DNA samples used for further studies.
• Mixed-mode separation modes may be exploited to optimize separations, including Lewis acid-base interactions, hydrophobic interactions and ion-exchange interactions. These modes may be attenuated by adjusting the strong Lewis base content, organic content and ionic strength of the mobile phase, respectively.
Legal Information    Discovery is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties
extent of labeling    25% Carbon loading
limit    <80 °C temp. range
L × I.D.    15 cm × 4.6 mm
matrix    zirconia spherical, porous particle platform
matrix active group    polyethyleneimine bonding phase
particle size    3 μm
pore size    300 Å
surface area    30 m2/g
operating pH range    1 - 12
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