主题:【求助】求助头疼死了,每天跟每天出的峰都不一样

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zhaojieyu2004
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溶液和背景都相同的配置,但不知为什么,基线前面都挺平的,快出峰时有很大的干扰峰,电流跟之前的一模一样,就是电泳图不一样,这是为什么呀?
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zhaojieyu2004
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就是电流重现性很好,但峰就是不一样,样品峰周围总有一个又宽又大的杂峰
poplar_yang
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zhaojieyu2004
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but when I deincrese my sample volume ,the peak is still very poor,why? Can you tell me,please!
sunpengwjh
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原文由 zhaojieyu2004(zhaojieyu2004) 发表:
but when I deincrese my sample volume ,the peak is still very poor,why? Can you tell me,please!

进样方式和进样量是多少?
poplar_yang
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it looks your sample is really complicated , with many different components. Plus this sample has different solvant , not similar as the buffer of your CE, right?
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zhaojieyu2004
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原文由 poplar_yang(poplar_yang) 发表:
it looks your sample is really complicated , with many different components. Plus this sample has different solvant , not similar as the buffer of your CE, right?
it didn't like that ,my sample is very simle ,it includes only one compent,because of stacking ,the sample diluent solution must be differt from BGE.
heguang
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zhaojieyu2004
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神鬼之约
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