For your 2nd problem, please also check your follow cell (air bubble, stain, etc.). If you have air bubble (long time no use) in the cell, light intensity from the D2 lamp will be much lower, give you the error message.
原文由 zlignc(zlignc) 发表: For your 2nd problem, please also check your follow cell (air bubble, stain, etc.). If you have air bubble (long time no use) in the cell, light intensity from the D2 lamp will be much lower, give you the error message.
But how to eliminati the bubble?Thanks for your guidance!
1. Create some back pressure after the dectector and suddenly release the pressure. Repeat this several time. 2. Flow pure organic solvent with how flow rate through your flow cell. 3. Use syringe to push methanol through the the flow cell.
You can try the above methods. You can also take out the flow cell to check the presend of bubble.