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原文由 qiwenss 发表:
1.check the solvent ,especilly check the millipore ,maybe the water have some problem .
2.wash the column with acetonitril ,maybe some sample stay in your column .
3.Sometimes when I use some HPLC ,the pressure is higher than average
pressure maybe 10 bar ,I think it is not a problem .
4.you can change the column to find there is problems in column or system .Sometimes ,there are some problem in inject needle or pump .
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