Table 1. $$lc gradient used for the analysis of ractopamine and clenbuterol.
Time (min) | A (%) | B (%) |
0 | 96 | 4 |
2 | 96 | 4 |
12 | 40 | 60 |
12.1 | 96 | 4 |
16.0 | 96 | 4 |
原文由 johne0212(johne0212) 发表:
先给你一张用Agilent 6460做的TIC图,两个物质前面是莱克多巴胺,后面是克伦布特罗.
1.1.1 HP$$lc conditions
Mobile phase A : 0.1 % formic acid in water (6.1.1)
Mobile phase B : 0.1 % formic acid in acetonitrile(6.1.2)
Injection volume : 2 µL
Column : Waters Acquity, BEH C18, 2.1 mm x 50 mm, 1.7 µm ID
Flow rate : 0.3 mL/min
Gradient : the $$lc gradient is described in Table 1
Column oven temperature : 30 °C
Autosampler temperature : room temperature
Diverter valve : the HP$$lc flow is directed into the MS detector between 3.0 and
9.0 min.Table 1. $$lc gradient used for the analysis of ractopamine and clenbuterol.
Time (min)A (%)
B (%)
0
96
4
2
96
4
12
40
60
12.1
96
4
16.0
96
4
原文由 johne0212(johne0212) 发表:
先给你一张用Agilent 6460做的TIC图,两个物质前面是莱克多巴胺,后面是克伦布特罗.
1.1.1 HP$$lc conditions
Mobile phase A : 0.1 % formic acid in water (6.1.1)
Mobile phase B : 0.1 % formic acid in acetonitrile(6.1.2)
Injection volume : 2 µL
Column : Waters Acquity, BEH C18, 2.1 mm x 50 mm, 1.7 µm ID
Flow rate : 0.3 mL/min
Gradient : the $$lc gradient is described in Table 1
Column oven temperature : 30 °C
Autosampler temperature : room temperature
Diverter valve : the HP$$lc flow is directed into the MS detector between 3.0 and
9.0 min.Table 1. $$lc gradient used for the analysis of ractopamine and clenbuterol.
Time (min)A (%)
B (%)
0
96
4
2
96
4
12
40
60
12.1
96
4
16.0
96
4
原文由 20100830(20100830) 发表:原文由 johne0212(johne0212) 发表:
先给你一张用Agilent 6460做的TIC图,两个物质前面是莱克多巴胺,后面是克伦布特罗.
1.1.1 HP$$lc conditions
Mobile phase A : 0.1 % formic acid in water (6.1.1)
Mobile phase B : 0.1 % formic acid in acetonitrile(6.1.2)
Injection volume : 2 µL
Column : Waters Acquity, BEH C18, 2.1 mm x 50 mm, 1.7 µm ID
Flow rate : 0.3 mL/min
Gradient : the $$lc gradient is described in Table 1
Column oven temperature : 30 °C
Autosampler temperature : room temperature
Diverter valve : the HP$$lc flow is directed into the MS detector between 3.0 and
9.0 min.Table 1. $$lc gradient used for the analysis of ractopamine and clenbuterol.
Time (min)A (%)
B (%)
0
96
4
2
96
4
12
40
60
12.1
96
4
16.0
96
4
您好,请问两个目标物混标后一起做,到时分析样品时怎么分析?是分别做两条曲线各自分析还是合着做一条曲线?在定量软件上应该怎么操作呢?