主题:【求助】求助,透射电镜的底片冲洗。

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弱弱的问一句,冲洗底片的步骤是什么?
我记得在大学的时候上课学的是
水洗,显影,水洗,定影,水洗,晾干。
但是现在我不确定了。
我想问问,在显影和定影中间的那步水洗是不是必须的步骤?为什么?
还有,显影过度的底片是不是还有救?谢谢大家的帮助。
推荐答案:sian回复于2006/12/20



好像没有传上,我复制,粘贴到下面吧

Developing of TEM Films

1. Preparation of Stock solution of D19 developer:

  Dissolve D19 in water according to the manufacturer's instructions

2. Development of KODAK film 4429:

    Dilute stock solution of D19 developer in water as follows:
    1 part of D19 + 2 parts of water

    Before use, check the temperature of D19 developer . It should be
    around 20 degrees centigrade.

3.  Developing time:

    2.5 minutes * with brief agitation every 30 seconds

4. Washing :

    Wash in diluted acetic acid solution ( 1:20 dilution)  for 30 seconds
   

5.  Fixing:

    Prepare Fixer solution  according to the manufacturer's instructions.
    Fix in the diluted solution for 2-5 minutes

6. Wash in running water for 20 minutes

7.  Dry the film

(*) The film development time depends on the emulsion setting of your
microscope. In our case, we use 2.5 minutes.


Developing of TEM films
补充答案:

怪味陈皮回复于2006/12/20

简单的说就是显影-水洗-定影-水洗-晾干,显影和定影之间的水洗是为了洗掉底片上的显影液。
试一下用Photoshop能不能救活显影过度的底片。

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sian
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我把师傅发给我的洗底片方法上传了。你看看有没用,我都是这样做的,效果很好
sian
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原文由 sian 发表:
我把师傅发给我的洗底片方法上传了。你看看有没用,我都是这样做的,效果很好



好像没有传上,我复制,粘贴到下面吧

Developing of TEM Films

1. Preparation of Stock solution of D19 developer:

  Dissolve D19 in water according to the manufacturer's instructions

2. Development of KODAK film 4429:

    Dilute stock solution of D19 developer in water as follows:
    1 part of D19 + 2 parts of water

    Before use, check the temperature of D19 developer . It should be
    around 20 degrees centigrade.

3.  Developing time:

    2.5 minutes * with brief agitation every 30 seconds

4. Washing :

    Wash in diluted acetic acid solution ( 1:20 dilution)  for 30 seconds
   

5.  Fixing:

    Prepare Fixer solution  according to the manufacturer's instructions.
    Fix in the diluted solution for 2-5 minutes

6. Wash in running water for 20 minutes

7.  Dry the film

(*) The film development time depends on the emulsion setting of your
microscope. In our case, we use 2.5 minutes.


Developing of TEM films
怪味陈皮
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简单的说就是显影-水洗-定影-水洗-晾干,显影和定影之间的水洗是为了洗掉底片上的显影液。
试一下用Photoshop能不能救活显影过度的底片。
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