主题：【求助】superdex peptide column, NaOH 洗后柱子缩掉了,所以重现性很差怎么办啊?

原文由 lynn2007 发表:
superdex peptide column, NaOH 洗后柱子缩掉了,所以重现性很差怎么办啊?
不知道有没有人用过类似的柱子？

如果有这种柱的胶，你可以试试重填或补填些胶。没有其它办法了。

根据该柱的说明用NaOH洗是没有问题的啊，如果是要用添胶的办法，那么就是没有办法挽救了～～

原文由 lynn2007 发表:
根据该柱的说明用NaOH洗是没有问题的啊，如果是要用添胶的办法，那么就是没有办法挽救了～～

一般来说：柱子可以用NaOH洗的话，在清洗方法中一定要有明确的NaOH的浓度，用量及作用时间、温度等参数。如果这些参数不明确，就不要用NaOH来清洗柱子，太危险了。

所使用NaOH是它的要求范围内,而且这个柱子对PH的耐受是1-14.开着空调25度,洗脱体积也按说明书要求一个柱体积左右.出现塌柱的情况,有点不明白?

如果你是买的成品柱，出现柱头塌陷应该很难恢复了，你是要分析蛋白质，氨基酸还是什么物质？ 下面是一个摘要供你参考。

The gel permeation behaviour of protein, small peptide and amino acid standards was assessed on a Superdex-Peptide column, using acetonitrile (30 or 70% containing 0.1% trifluoroacetic acid, TFA) as mobile phase. Efficient separation of the standards was observed using 30% acetonitrile; however, non-ideal gel permeation effects were observed for some charged amino acids and peptides containing hydrophobic residues. Retardation of the hydrophobic peptide standards was significantly reduced/eliminated using 70% acetonitrile as the mobile phase. However, chromatography with 70% acetonitrile was not suitable for molecular mass determination of proteins and free amino acids. The chromatographic behaviour of hydrolysates of β-lactoglobulin (β-Ig) and whey protein concentrate on Superdex-Peptide and on TSK-2000SW columns was compared. Better separation of the hydrolysate peptides was obtained on the Superdex-Peptide column.

如果是你自己装填的，就从新装一次了。
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