给你一个你可以参考一下:
色谱柱:HYPERSII. C18,5μm,250mm?4.6mm I.D.
流动相:A.乙腈(0.1%乙酸);B.水(0.1%乙酸)
梯度条件:
O一48min,50% A;
48- 68min,50%一65% A;
68-95min,65%-75%A
柱温:25℃
流速:1.OmL/min
检测器:DAD,242nm;
ESI进样量:20μL
具体色谱图参考:http://www.chemalink.net/ChromGall/html/584b76697c149b43.html