高效[url=https://insevent.instrument.com.cn/t/5p]液相色谱法同时测定多种食品添加剂[/url]
【摘要】 采用反相高效
液相色谱法 ,一次进样、同时测定食品中的人工合成甜味剂 (糖精钠、安赛蜜、甜味素 )、防腐剂(苯甲酸、山梨酸 )、咖啡因、可可碱和茶碱。以AlltechEconosphereC18柱 (15 0mm× 4 6mmi.d .,3μm)为分离柱 ,10mmol/LNaH2 PO4 (pH 4 0 0 ) 乙腈 (体积比为 90∶10 )为流动相 ,采用二极管阵列检测器进行检测。整个分离过程在 2 3min内完成。样品平均加标回收率为 78 5 %~ 10 7 2 %。方法也可用于药品分析
【英文摘要】 A novel method is proposed for the simultaneous separation and determination of eight food additives, acesulfame potassium (AK), aspartame (ASP), benzoic acid (BA), caffeine (CA), saccharin sodium (SA), sorbic acid (SOR), theobromine (TB) and theophylline (TP) by reversed phase high performance liquid chromatography. The separation was achieved within 23 min by using an Alltech Econosphere C 18 column with 10 mmol/L NaH 2PO 4 (pH 4.00) acetonitrile (90∶10, V/V ) as mobile phase. The qualification...