主题：【讨论】有没有人做过:三甲基氧苄二氨嘧啶?

楼主，你好！三甲基氧苄二氨嘧啶的英文名称是“Trimethoprim”吧？如果是的话，您可以看看下面的两篇英文文献的摘要，需要的话可以到文献求助版面索求全文，这是在ScienceDirect数据库中的。中文的文献没有找到相关的。
Solid phase microextraction of macrolide, trimethoprim, and sulfonamide antibiotics in wastewaters

Evelyn L. McClurea and Charles S. Wong, a, 
aDepartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada
Received 25 June 2007;  revised 27 August 2007;  accepted 30 August 2007.  Available online 1 September 2007.

Abstract
In this work, we optimize a solid phase microextraction (SPME) method for the simultaneous collection of antibiotics (sulfonamides, macrolides, and trimethoprim) present in wastewaters. The performance of the SPME method is compared to a solid phase extraction (SPE) method. Analytes in both cases were quantified by high performance liquid chromatography–tandem mass spectrometry (HPLC–MS–MS) with electrospray ionization. The advantages offered by SPME in this application are: decreased sample volume requirements, ease of sample processing and extraction, decreased cost, and most importantly, elimination of electrospray matrix effects. Despite having higher limits of quantification (16–1380 ng/L in influent and 35–260 ng/L in effluent), nearly all of the compounds found to be present in Edmonton Gold Bar wastewater by SPE were measurable by SPME (i.e., sulfamethoxazole, trimethoprim, erythromycin, and clarithromycin), with values similar to those obtained using the former method. Limits of quantification for the SPE method for the measured compounds were 4.7–15 ng/L and 0.86–6.1 ng/L for influent and effluent, respectively.

Keywords: Solid phase microextraction; Solid phase extraction; Antibiotics; Wastewater; Matrix effects

Simultaneous determination of sulfamethoxazole and trimethoprim in biological fluids for high-throughput analysis: Comparison of HPLC with ultraviolet and tandem mass spectrometric detection

D.C.G. Bedora, , , T.M. Gonçalvesa, M.L.L. Ferreiraa, C.E.M. de Sousaa, A.L. Menezesa, E.J. Oliveirab and D.P. de Santanaa, 
aNúcleo de Desenvolvimento Farmacêutico e Cosmético, Rua Professor Arthur de Sá s/n, Cidade Universitária, CEP 50740-520, Recife, Pernambuco, Brazil
bLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba (LTF-UFPB), Brazil
Received 15 March 2007;  accepted 24 December 2007.  Available online 17 January 2008.

Abstract
The comparison of two methods based on online solid phase extraction–liquid chromatography with UV (SPE–LC–UV) or mass spectrometry detection (SPE–LC–MS/MS) for the simultaneous quantification of sulfamethoxazole (SMZ) and trimethoprim (TMP) is presented. The methods were validated and proved to be accurate. The analysis of standard samples for SMZ at concentrations of 0.5, 1.5, 25 and 50 μg/mL demonstrated a relative standard deviation of less than 6% for both methods (n = 18), while TMP samples at concentrations of 0.05, 0.15, 1.5 and 5.0 μg/mL were analyzed with R.S.D. of less than 4% (n = 18). The method with mass spectrometric detection was approximately six times more sensitive than the method with ultraviolet detection. The total run time for the SPE–LC–MS/MS was 2.5 min per sample as opposed to 18.0 min for the SPE–LC–UV method. The method with MS detection in comparison with UV detection proved to be more rugged and was successfully applied to pharmacokinetics studies.

Keywords: Sulfamethoxazole; Trimethoprim; High-throughput; SPE–LC–MS/MS; SPE–LC–UV

TMP是兽药,用液相做,,这个药是增效剂吗???

C18柱...流动相条件和柱子要配好,很久前做过..只记得这些了