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原文由 userwzy(userwzy) 发表:
Use acetone as a marker, and calculate the EOF according to the migration time, column length and voltage.
If the EOF is very low, use 3 injection method. First, injection No.1 then flash with low pressure for several minutes, and then injection No. 2 and flash with some pressure and time, and add a volatege migrate several minutes, at last injection No. 3, then flush the column until the 3 peaks were shown. At last, according the distance difference between peak 1 and 2 and peak 2 and 3, migrate time and voltage calculte the EOF. it''s an old paper, I think you can find it.
原文由 guocc007(guocc007) 发表:原文由 windeast(windeast) 发表:
一般是丙酮的，十年前额就是用这个标记淌度，叫做电渗流标记物。
通常的研究都是用时间来进行比较和表征的，所以有个t=tm-t0的公式，这个是套用的色谱的。
李发美这小子还越做越发迹了。不错 哈哈。
呵呵，李发美是位女老师，一位老太太
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