与峰重叠，我试过了好多C18的柱子，都是重叠的。我想问问，对于这两种化合物，根据结构应该选取什么柱子会好一点，或者在选用C18柱子流动相该如何考虑。谢谢。 Solution A— Prepare a mixture of Diluted acetic acid, methanol, and acetonitrile (60:35:5). Solution B— Prepare a mixture of methanol, Diluted acetic acid, and acetonitrile (80:15:5). chromatograph is equipped with a 250-nm detector, a refrigerated autosamplerat 5, and a 4.6-mm × 25-cm column that contains5-µm packing L1. The flow rate is about 1 mL per minute. The column temperatureis maintained at 40. The chromatograph is programmed as follows.
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峰重叠，我试过了好多C18的柱子，都是重叠的。我想问问，对于这两种化合物，根据结构应该选取什么柱子会好一点，或者在选用C18柱子流动相该如何考虑。谢谢。
, and a 4.6-mm × 25-cm column that contains5-µm packing L1. The flow rate is about 1 mL per minute. The column temperatureis maintained at 40
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