主题：【分享】27.6 HPLC法测定风湿定片中毒藜碱的含量

作者】 梁从庆； 李书渊； 房志坚；

【Author】 LIANG Congqing,LI Shuyuan,FANG Zhijian(Guangdong College of Pharmacy,Guangzhou 510224)

【摘要】 目的测定风湿定片中毒藜碱的含量。方法采用反相高效液相色谱法,Diamonsil-C18柱,甲醇-0.1%三乙胺(5∶95,磷酸调节pH至3.6)为流动相,流速为1.0mL·min-1,检测波长259nm,柱温为室温。结果毒藜碱在0.956～5.76μg范围内线性关系良好,r=0.9999;毒藜碱平均回收率为99.0%,RSD=2.8%(n=6)。结论该方法可用于风湿定片中毒藜碱的含量测定。 更多还原

【Abstract】 Objective To determine the content of anabasine in Fengshiding tablets.Methods RP-HPLC method was established.Diamonsil-C18 was used as chromatographic column,the mobile phase consisted of methanol and 0.1 %triethylamine(5 ∶95,adjust pH to 3.6 with H3PO4),the flow rate was 1.0 mL·min-1 and the detection wavelength at 259 nm.Results The average recovery for Anabasine was 99.0 %,RSD=2.8 %(n=6).Conclusion The method can be used to determine the content of anabasine in Fengshiding tablets. 更多还原

【关键词】 风湿定片； 毒藜碱； HPLC法； 含量测定；

【Key words】 Fengshiding tablets； Anabasine； HPLC； Content determination；