40 Pesticides out of a multi-target screening method for 300 compounds
色谱柱:Synergi™ 4 µm Fusion-RP 80 Å, $$lc Column 50 x 2 mm, Ea
货号:00B-4424-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:H2O + 5mM NH4formate
洗脱B:CH3OH + 5mM NH4formate
梯度:
步骤顺序
时间 (分)
洗脱A
洗脱B
1
0
80
20
2
11
10
90
3
23
10
90
流速:0.2 mL/min
冷却温度:20 °C
检测:Mass Spectrometer (MS) @ amu (22°C)
Detector Info:AB Sciex 4000 QTRAP® $$lc/MS/MS system, Turbo V, source with TurboIonSpray® probe TEM = 450°C, IS= 5000V, Declustering Potential (DP) = 30V, Collision Energy (CE) = optimized for each compound Positive polarity, Multiple Reaction Monitoring of 297 transitions (MRMs of example see table)
Information Dependent Acquisition of 3 Enhanced Product Ion spectra to confirm detected compound at CE = 20, 35, and 50V, Dynamic fill time to avoid linear ion trap saturation, Dynamic background subtraction to allow detection of co-eluting peaks, Mass spectral library searching
备注:SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Fusion-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-7556 - Holder Part No.: KJ0-4282
Screening of 535 Pesticides by QuEchERS and $$lc/MS/MS
色谱柱:Synergi™ 2.5 µm Fusion-RP 100 Å, $$lc Column 50 x 2 mm, Ea
货号:00B-4423-B0
尺寸:50 x 2 mm ID
洗脱类型:Gradient
洗脱A:Water/Methanol (90:10) + 5 mM ammonium formate
洗脱B:Methanol/Water (90:10) + 5 mM ammonium formate
梯度:
步骤顺序
时间 (分)
洗脱A
洗脱B
1
0
100
0
2
1
100
0
3
15
0
100
4
18
0
100
5
18.05
100
0
6
20
100
0
流速:0.4 mL/min
冷却温度:40 °C
检测:Tandem Mass Spec (MS-MS) @ (400°C)
Detector Info:AB Sciex API 3200™
备注:Phenomenex SecurityGuard Guard Cartridge kit (P/N KJ0-4282) with C18 4 x 2.0 mm cartridge (P/N AJ0-4286)
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