主题：【求助】求TSK Gel 2000SWxl 排阻范围

原文由 binbiozb 发表:
如题
有用过这条柱子的老师帮个忙
TSK Gel 2000SWxl 排阻范围

非常感谢

排阻色谱，还没用过呢

哦，查到了
3W以下~

这个型号色谱柱的排阻界限：100000（蛋白质）
下面是其他使用的相关信息

A.    OPERATING CONDITIONS
   
01.    Shipping Solvent:    0.05% NaN3 and 0.1M Na2SO4 in 0.1M phosphate buffer, pH 6.7

02.    Max. Flow Rate:    1.2 mL/min

    When a buffer with high viscosity is used, the maximum flow rate may have to be reduced so as not to exceed the maximum pressure drop.  When changing solvents, use a flow rate equal to 25% of the maximum flow rate.

03.    Standard Flow Rate:    0.5 - 1.0 mL/min

04.    Max. Pressure:    70 kg/cm2 = 1050 psi

05.    pH Range:    2.5 - 7.5

06.    Salt Conc.:    * 0.5 Molar

07.    Organic Conc.:    0 - 100% for aqueous soluble organic solvents.  Make gradual solvent changes using a shallow gradient at low flow rate.

08.    Temperature:    10 - 30°C.  Reduce flow rate when operating below 10°C.

09.    Cleaning Solvents:



        NOTE:    (1)  conc. salt solution at low pH, e.g. 0.5M Na2SO4, pH 2.7
(2)  methanol or acetonitrile in low conc. aqueous buffer
(3)  buffered solution of SDS, urea or guanidine

Choose a cleaning solvent based on sample properties, e.g. use (1) to remove basic proteins, and (2) to remove hydrophobic proteins.  Chaotrophic agents can solvate strongly adsorbed proteins, e.g. via hydrogen bonding.

10.    Storage:    Store the column in mobile phase containing 0.05% NaN3or 20% ethanol  when it will not be used the next day.  For overnight storage flush the column with mobile phase at low flow rate.  Prevent air from entering the column!

11.    Column Protection:    The use of guard columns is recommended to prolong the life of the analytical column.  Guard column life depends greatly on sample cleanliness.  As a general rule, guard columns should be replaced after every 30-40 sample injections, when the peaks become excessively wide, or when the peaks show splitting.

原文由 binbiozb 发表:
如题
有用过这条柱子的老师帮个忙
TSK Gel 2000SWxl 排阻范围

非常感谢

粒度：5μm；
孔径：125A；

分离范围：
球蛋白：5,000～150,000
聚乙二醇：500～15,000

500-100000