PAH metabolites were derivatized with 20 μL of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA, Fluke, Buchs, Switzerland). Extracts were analyzed by GC-MS to quantify PAHs and their metabolites.
Analysis byGC-MSwas performed with a gas chromatograph (HP-6890) coupled to a 5973 C quadrupole mass spectrometer (Agilent Technologies,Massy, France). The split/splitless injector was maintained at 280 C, and the injection volume was 2 μL. The column used for separating PAHand their metabolites was a OV-1 (Ohio-Valley) (30 m 0.25 mm inner diameter; film thickness, 0.25 μm). The temperature gradient was 110 C (4.5min), 20 C/min until 160 C, 15 C/min until 300 C (10 min hold), and then 20 C/min until 320 C (2 min). Analyte ionization was performed by electron ionization (70 eV), and signal acquisition was realized in the single ion monitoring (SIM) mode. Separate analyte determination was performed for parent PAHs and hydroxylated metabolites.
PAH metabolites were derivatized with 20 μL of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA, Fluke, Buchs, Switzerland). Extracts were analyzed by GC-MS to quantify PAHs and their metabolites.
Analysis byGC-MSwas performed with a gas chromatograph (HP-6890) coupled to a 5973 C quadrupole mass spectrometer (Agilent Technologies,Massy, France). The split/splitless injector was maintained at 280 C, and the injection volume was 2 μL. The column used for separating PAHand their metabolites was a OV-1 (Ohio-Valley) (30 m 0.25 mm inner diameter; film thickness, 0.25 μm). The temperature gradient was 110 C (4.5min), 20 C/min until 160 C, 15 C/min until 300 C (10 min hold), and then 20 C/min until 320 C (2 min). Analyte ionization was performed by electron ionization (70 eV), and signal acquisition was realized in the single ion monitoring (SIM) mode. Separate analyte determination was performed for parent PAHs and hydroxylated metabolites.
PAH metabolites were derivatized with 20 μL of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA, Fluke, Buchs, Switzerland). Extracts were analyzed by GC-MS to quantify PAHs and their metabolites. LODand LOQ determined for PAHs and theirmetabolites in urine are presented in Table 2. Quantification ofPAHsand Metabolites byGC-MS.Analysis byGC-MSwas performed with a gas chromatograph (HP-6890) coupled to a 5973 C quadrupole mass spectrometer (Agilent Technologies,Massy, France). The split/splitless injector was maintained at 280 C, and the injection volume was 2 μL. The column used for separating PAHand their metabolites was a OV-1 (Ohio-Valley) (30 m 0.25 mm inner diameter; film thickness, 0.25 μm). The temperature gradient was 110 C (4.5min), 20 C/min until 160 C, 15 C/min until 300 C (10 min hold), and then 20 C/min until 320 C (2 min). Analyte ionization was performed by electron ionization (70 eV), and signal acquisition was realized in the single ion monitoring (SIM) mode. Separate analyte determination was performed for parent PAHs and hydroxylated metabolites.
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