Abstract Objective: Through the comparision of the two methods for extract the Tea Polyphenols in Trilobita Tea and the research for separation method obtain the best extraction craft and the preliminary separation method; Method: Adds 80% alcohol of 10 times raw material amounts, the microwave extract 2 times, the reduced pressure distillation recycling ethyl alcohol, pulls out filters, respectively extracted by the benzene, the chloroform, the ethyl acetate, after the reduced pressure distillation recycling ethyl acetate, crossed the Polyamide chromatographic analysis column, eluted by the different gradient ethyl alcohol, the fluid through reduced pressure concentration, crossed the Silica gel chromatographic analysis column, elutes by the different polarity fluid; Results: Can get more unitary component basically; Conclusion: The effect of the method for extraction,isolationz Tea Polyphenol in Trilobita tea is fairly good.