引用： -------------------------------------------------------------------------------- 原文由 qiwenss 发表: 1.check the solvent ,especilly check the millipore ,maybe the water have some problem . 2.wash the column with acetonitril ,maybe some sample stay in your column . 3.Sometimes when I use some HPLC ,the pressure is higher than average pressure maybe 10 bar ,I think it is not a problem . 4.you can change the column to find there is problems in column or system .Sometimes ,there are some problem in inject needle or pump . --------------------------------------------------------------------------------
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