主题：【讨论】气质联用做香气分析，加内标如何定量？

原文由 liumancang(v2912500) 发表:
校正因子是1 有什么依据吗？
如果做这种半定量，任何一台机器的校正因子（在没法测得的情况下） 都可视为1吗？

原文由 liumancang(v2912500) 发表:
我也是用这种半定量方法做的植物挥发物，审稿人提出了如下问题，如下：
Can you explain your quantification further? If you compared a volatiles peak area to that of a dose response curve of a known amount of the standard of that specific compound then this is fine. But if you just looked at ratios to a single internal standard then I do not believe this is appropriate as the MS will have different sensitivity to different compounds. If the later then it is better to report peak area than quantities that will not be accurate.

该如何回复？

原文由 花开见我(cai008) 发表:
用癸酸乙酯内标，校正因子假设为1，那会不会前面出峰的物质，偏差比较大。

原文由 liumancang(v2912500) 发表:
我也是用这种半定量方法做的植物挥发物，审稿人提出了如下问题，如下：
Can you explain your quantification further? If you compared a volatiles peak area to that of a dose response curve of a known amount of the standard of that specific compound then this is fine. But if you just looked at ratios to a single internal standard then I do not believe this is appropriate as the MS will have different sensitivity to different compounds. If the later then it is better to report peak area than quantities that will not be accurate.

该如何回复？

原文由 symmacros(jimzhu) 发表:
不一定是前面出峰的物质，主要是物质的性质而定。

原文由 花开见我(cai008) 发表:
也想做内标定量方面的工作，不过可能工作量很大，而且内标物选择方面还要摸索。

原文由 symmacros(jimzhu) 发表:
没有标样，要比较准确的定量就难了。请问是测定单个样品呢？还是比较几个样品呢？

原文由 千层峰(jxyan) 发表:
买氘代物，贵。不用摸。。