Conditions for solution stability have also been de- veloped.17 This method would be useful for assaying the stability of compounds in pH buffers, assay buffers, cell culture buffers, and simulated gastric intestinal fluids. Advantage can be taken of the ability of some HPLC autosampler syringes to add solutions of reagents to samples in well plates in the autosampler and to incubate the samples at a specified temperature (e.g., 37 C). The autosampler is programmed to inject aliquots of this reaction mixture into the HPLC at specified time points, in order to calculate the reaction kinetics. Again, the automation of the autosampler system and software allows unattended experiments over many hours or days for large numbers of samples.
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