主题：【求助】液相色谱峰突然拖尾严重，高手快来呀

风烟回复于2008/06/08

估计如1楼老师所讲，导致您的柱子有了问题，所以才会出现这样的峰。
现在的话，先冲洗柱子吧：
　　用乙腈冲柱子，在您平时的流速的一半左右，想必要比较长的时间，把缓冲盐冲干净后，再用吧。

　　如果冲它个一两天还是不见效果，就倒过来冲洗，接着倒着使用吧，像这样的情况，倒着冲应该是有效果的。

hrcren回复于2008/06/08

就是跟三楼的附件说的一样，加入一定量的三乙胺或醋酸能够抑制HPLC峰拖尾

maxtall回复于2008/06/08

the file from upstair is great:

Correcting Peak Tailing Problems in Reversed Phase HPLC
Peak tailing in reversed phase HPLC continues to be a common complaint. It is particularly prevalent when
separating basic compounds and, therefore, a source of constant problems to those analyzing pharmaceutical
compounds by HPLC.
Peak tailing causes a number of problems, including lower resolution, reduced sensitivity, and poorer
precision and quantitation. Figure 1 illustrates how resolution between peaks and sample sensitivity is
negatively affected by peak tailing. Figure 2 illustrates how accuracy and precision of an analysis can suffer
because of the inability of data systems to identify exactly where a tailing peak begins and ends.