原文由 happyjyl 发表:
Mobile phase: buffered salt solution (0.01mol/L KH2PO4 and 0.005mol/L EDTANa2, adjusted to pH4.0 with phosphoric acid)-acetonitrile (80:20)
Flow rate: 1.0ml/min
Oven temp.: 35℃
Wavelength: 275nm
Sample injection volume: 60µL
Inject the standard solution and test solution respectively.