Find the λmax for the compound. Use UV detector at λmax. Please note that λmax depends on pH. As for HPLC conditions, try 4.6x150 mm HPLC column with gradient elution. Flow rate 1 mL/min.
MP A: 0.05 M NH4OAc in Water MP B: 0.05 M NH4OAc in MeOH
Time (min) 0 8 10 10.01 13 % B 30 90 90 30 30
This is just something for you to start with. You can take the next step based on the data from the initial test.
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