原文由 poorlittle(poorlittle) 发表:
From the original: (http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.1998.00991.x/full)
Glucose is a sugar transported by the phosphotransferase system (PTS) and can enter the cell by either of two transporters: the glucose-specific EIIBC, product of the ptsG gene, located at 25 min on the E. coli chromosome; and the so-called mannose PTS, EIIAB, IIC, IIDMan encoded by the manXYZ genes (previously ptsML ) located at 41 min.
Since “41 min” also appears in the original, it is unlikely that these figures denote the orientation.
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LZ is suggested to search with the key words “Glucose, min” (http://www.google.com.hk/#hl=zh-TW&source=hp&q=+%E2%80%9CGlucose%2C+min%E2%80%9D&btnG=Google+%E6%90%9C%E5%B0%8B&rlz=1R2ADRA_enHK408&oq=+%E2%80%9CGlucose%2C+min%E2%80%9D&aq=f&aqi=&aql=1&gs_sm=s&gs_upl=1771l1771l0l3265l1l1l0l0l0l0l495l495l4-1l1l0&bav=cf.osb&fp=c344195479ec8e10&biw=1280&bih=542
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From http://www.horizon-scientific-press.co.uk/abs/abspts.html :
There are three main routes for the utilization of fructose by Escherichia coli. One (Route A) predominates in the growth of wild-type strains. It involves the functioning of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) and a fructose operon, mapping at min. 48.7, containing genes for a membrane-spanning protein (fruA), a 1-phosphofructose kinase (fruK) and a diphosphoryl transfer protein (fruB), under negative regulation by a fruR gene mapping at min. 1.9.
Does “mapping at min. xxx” make sense ?
Would the “n” in “min” denote Nanomoles ?
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If LZ has the answer, please tell us.
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I don't know how to post a picture here. You may try searching a picture of "circular genetic map" for E. Coli, then you'll understand.