主题：【资料】HPLC在肽类及蛋白类物质分析中应用（原版电子书）

HPLC在肽类及蛋白类物质分析中应用（原版电子书）
书名：HPLC of Peptides and Proteins：Methods and Protocols
主编：Marie-Isabel Aguilar
出版社：Humana Press
时间：2004
目录：
PART I MODES OF HPLC
1 HPLC of Peptides and Proteins:
Basic Theory and Methodology ................................................ 3
2 Reversed-Phase High-Performance Liquid Chromatography ..... 9
3 Ion-Exchange Chromatography .................................................. 23
4 High-Performance Hydrophobic Interaction Chromatography... 45
5 Gel Filtration Chromatography .................................................... 55
6 Hydrophilic Interaction Chromatography .................................... 75
7 Immobilized Metal Ion Affinity Chromatography of Proteins ...... 89
8 Immunoaffinity Chromatography of Proteins ............................ 103
9 Liquid Chromatography–Mass Spectrometry and Tandem
Mass Spectrometry of Peptides and Proteins ...................... 111
PART II PREPARATIVE METHODOLOGIES
10 Capillary Separations ................................................................ 143
11 Micropreparative HPLC of Peptides and Proteins ................... 165
12 Multidimensional HPLC Purification of Proteins ....................... 177
13 Analytical High-Performance Liquid Chromatography ............. 183
14 Prep/Semiprep Separations of Peptides .................................. 191
15 Large-Scale Protein Chromatography ...................................... 211
PART III APPLICATIONS
16 Proteolytic Peptide Mapping ..................................................... 227
17 Mass Spectrometric Characterization of Posttranslationally
Modified Proteins—Phosphorylation..................................... 245
18 Analyses of Glycopeptides and Glycoproteins
by Liquid Chromatography–Mass Spectrometry
and Liquid Chromatography–Tandem Mass Spectrometry .... 263
19 HPLC in the Analysis of Peptide Metabolism ........................... 275
20 Isolation and Characterization of Naturally Processed
MHC-Bound Peptides From the Surface
of Antigen-Presenting Cells .................................................. 291
21 Australian Funnel-Web Spider Venom Analyzed
With On-Line RP-HPLC Techniques .................................... 307
22 HPLC and Mass Spectrometry of Intrinsic Membrane
Proteins .................................................................................. 323
23 IgG Purification .......................................................................... 341
24 DNA–Binding Proteins: LC–MS to Identify Key Domains
in RNA Polymerase–Promoter Interactions .......................... 351
25 Sensitive Enzymatic Analysis of Histidine Decarboxylase
Using HPLC ........................................................................... 365
26 Automated vs Manual Profiling of Peptide Libraries
by Mass Spectrometry ........................................................... 377
27 Proteome Analysis .................................................................... 387
Index ................................................................................................. 401

HPLC of Peptides and Proteins：Methods and Protocols