主题：【求助】农残检测新方法

家里检测基本上比较困难，原来在《科技新时代》杂志上见到国外一种商品化的试纸，是利用乙酰胆碱酯酶的原理检测有机磷类农药，几个美金一片吧。

原文由 watericecat 发表:
家里检测基本上比较困难，原来在《科技新时代》杂志上见到国外一种商品化的试纸，是利用乙酰胆碱酯酶的原理检测有机磷类农药，几个美金一片吧。

估计在中国一般人是用不起了。一片一二十块，蔬菜也不值这个钱。富人专用。

楼主，你好！建议您到文献求助应助版进行求助：
全文的链接如下：  http://www.impublications.com/NIR/abstract/J15_0227

ABSTRACT:
In a previous article, a pesticide determination system using near infrared (NIR) spectroscopy and the dry-extract system for infrared (DESIR) technique had been developed. In order to evaluate system reliability, a number of tests had been conducted. To reduce time and labour needed for pesticide assays by gas chromatography, artificial solutions of each pesticide in acetone were used in place of sample extract that was used in the previous article. Effects of several factors, such as chemical structure, interference of another reagent in the solution and sample presentation, on the system accuracy were evaluated. A tentative collaborative study was conducted to evaluate the possibility of large scale utilisation. To test the influence of chemical structure, three pesticides—acephate, dichlofluanid and tetrachloro-isophthalonitrile (TPN)—having different numbers of functional groups with strong dipole moment were used. From the range of 0 ppm to 50 ppm active ingredient in acetone, the SEPs obtained were 2.1, 5.3 and 9.3 ppm for acephate, dichlofluanid and TPN, respectively. These results corresponded to the number of strong dipole moment groups in the chemical structure which were four for acephate, two for dichlofluanid and none for TPN. In the case where two kinds of pesticide were presented in the system, the SEP became larger compared to the single pesticide results. The degree of interference differed depending on the relative absorptivity between the target pesticide and the interference. Using the system developed and acephate solution, a tentative collaborative study was conducted using three laboratories and four technicians. The almost similar SEPs of 2.8, 2.8, 3.0 and 2.5 ppm were obtained by the four technicians, indicating that even if the NIR instruments used and the degree of professional skill differed between technicians, satisfactory results could be obtained after a few hours of training and a proper bias correction. Finally, to simplify the system, three kinds of sample presentation were used to develop a calibration equation for acephate. The SEPs obtained differed only minutely. It could be concluded that when using NIR analysis the operator may choose between the highly precise system which requires more time and labour because of the sample preparation involved or a slightly less precise system with simple sample presentation. Based on the Japanese pesticide control level, the developed system could be used for the monitoring of some pesticides in fruits and vegetables.


Keywords: pesticide, near infrared spectroscopy, NIR, DESIR, chemical structure, collaborative study, sample presentation, ppm

常规方法还是用紫外分光光度计，而且不需要太高档的。当然如果机器或者软件本身带有农产检验卡或快速方法的话就方便很多了。这种仪器很多。另外还有试剂可以检验，我知道有一种德国必发试剂，有农残检验试剂，快速方便。但肯定不如仪器精确。建议可以去你们当地的农产品技术推广站看看他们实验室的仪器和方法。