4.测定法
建立……测定的HPLC法
develop an HPLC method for determination of ……
将上述溶液取20μl注入
液相色谱仪Inject 20 μl of the resulting solution into the column
另取……对照品,同法测定。
Repeat the operation,using … CRS instead of the substance being examed.
理论塔板数按……峰计算应不低于1000。
The number of theoretical plates of the column is not less than 1000, calculated with reference to the peak of ….
记录色谱图
record the chromatogram
record the peak areas correspondingly obtained in the chromatogram
照……项下的方法进行含量测定
Carry out the Assay described under …
照高效
液相色谱法(附录Ⅴ D)测定
Carry out the method for high performance liquid chromatography (Appendix Ⅴ D)